Question 1

What are Branched Amphipathic Peptide Capsules (BAPC®️)?

Accepted Answer

BAPC®️ are nanospheres made from two branched peptides that self-assemble to form solvent-filled, bilayer defined capsules. They serve as nano-delivery vehicles that overcome many of the poor absorption issues that plague certain classes of drugs: vaccines, therapeutics, diagnostics, pesticides, etc.

Question 2

How small are BAPC®️?

Accepted Answer

BAPC®️ are produced to specification in sizes ranging from six nanometers to two micrometers.

Question 3

Why would I need different size BAPC®️?

Accepted Answer

The optimal size needed depends on the application. For cell transfection research, BAPC®️ are available in the following sizes: 6, 10, 25, and 50 nanometers. Larger BAPC®️ can be prepared and can be used for encapsulating larger volumes for delivery of active ingredients such as fungicides.

Question 4

How is the surface charge affected by addition of nucleic acid particles (i.e. iRNA and dsRNA)?

Accepted Answer

The positive surface charge is decreased due to electrostatic attractions but not to a point where their net charge becomes negative

Question 5

Can BAPC®️ be customized for my specific application?

Accepted Answer

Yes. We realize that all research has unique considerations, so we can customize BAPC®️ to your exact specifications. If you’re not sure which size BAPC®️ are best suited for your research, we invite you to contact our team for a consultation.

Question 6

What components can BAPC®️ be used with?

Accepted Answer

The composition of BAPC®️ allows hydrophilic small molecules, proteins and nucleic acids to be either surface bound or encapsulated within its compartment during the assembly process. This works both in vitro and in vivo.

Question 7

What can BAPC®️ encapsulate?

Accepted Answer

BAPC® can encapsulate the following: fluorescent dyes, therapeutic drugs, radionuclides, pesticides, fungicides, colloidal gold, and magnetic beads.

Question 8

What can be bound to the surface of BAPC®️?

Accepted Answer

The following can be bound to the surface of BAPC®: proteins, sugars, dsRNA, siRNA, oligonucleotides, plasmids, and CRISPR-Cas9 components

Question 9

Are BAPC®️ toxic?

Accepted Answer

While many nanoparticle carriers are toxic to target cells, BAPC®️ are non-toxic due to their natural peptide composition, stability and slightly positive surface charge.

Question 10

What commercial applications can BAPC®️ be used for?

Accepted Answer

BAPC®️ are an adaptable carrier platform with applications for multiple industries including medicine, agriculture, and research.

Question 11

What is BAPtofect®️-25?

Accepted Answer

BAPtofect®️-25 kits are 25 nm conformationally locked BAPC in quantities of 0.5mg, 1.0 mg, 5 mg and 10 mg for use in cell transfection research. 25 nm BAPC®️ have been proven to be the ideal size to achieve maximum cell transfection rates.

Question 12

How does BAPtofect®️-25 work for cell transfection?

Accepted Answer

BAPC®️ enter cells via the endosomal pathway providing targeted, protected delivery of their payloads. Both surface-bound proteins and nucleic acids are shielded from lytic degradation.

Question 13

What is the surface charge of BAPtoFect®️-25?

Accepted Answer

+25 to 30 mV

Question 14

How is BAPC®️ shipped?

Accepted Answer

US shipments are sent via USPS Priority Mail for delivery within one to three days. International shipments are sent via Federal Express Economy unless expedited delivery is requested.

Question 15

How is BAPC®️ stored?

Accepted Answer

BAPC®️ should be stored in a cool and dry location. BAPC®️ with added fluorescent dye should also be stored in a dark location.

FAQs