Research Data

Abstract: CRISPR/Cas9 gene editing is a powerful technology to study the genetics of rising model organisms, such as the jewel wasp Nasonia vitripennis. However, current methods involving embryonic microinjection of CRISPR reagents are challenging. Delivery of Cas9...

Abstract: A new method BAPC-assisted-CRISPR-Delivery System, improved heritable gene editing when injecting adults or nymphs of insects [Hemiptera]. Addition of Branched Amphiphilic Peptide Capsules, BAPCtofect™ (Phoreus™ Biotechnology, Inc.,) improved delivery of CRISPR components for heritable gene...

Abstract: We provide strong chemical and biophysical evidence that documents that branched amphiphilic peptides, BAPs, known to assemble into spherical nanoassemblies in solution, do assemble as peptide-bilayer-delimited capsules. These nanoassemblies are termed branched amphiphilic peptide capsules...

Abstract: This chapter will discuss cationic polymers containing ɛ-amine groups and immine or amide linkages that are being used as nanocarriers for nonviral gene delivery. We will focus particularly on cationic peptides. Cationic peptides, free or complexed...

Abstract: Branched amphipathic peptide capsules (BAPCs) are biologically derived, bilayer delimited, nanovesicles capable of being coated by or encapsulating a wide variety of solutes. The vesicles and their cargos are readily taken up by cells and...

Abstract: A class of self-assembling branched amphiphilic peptide capsules (BAPCs) was recently developed that could serve as a new drug delivery vehicle. BAPCs can encapsulate solutes up to ∼12 kDa during assembly, are unusually stable, and...

Abstract: Various strategies are being developed to improve delivery and increase the biological half-lives of pharmacological agents. To address these issues, drug delivery technologies rely on different nano-sized molecules including: lipid vesicles, viral capsids and nano-particles....

Abstract: Branched amphiphilic peptide capsules (BAPCs) are biocompatible, bilayer delimited polycationic nanospheres that spontaneously form at room temperature through the coassembly of two amphiphilic branched peptides: bis(FLIVI)-K-K4 and bis(FLIVIGSII)-K-K4. BAPCs are readily taken up by cells...

Abstract: In a recent article (Gudlur et al. PLOS ONE, 2012, 7 (9) e45374), we described the special properties of a mixed branched peptide assembly in which equimolar bis(FLIVI)-K-KKKK and bis(FLIVIGSII)-K-KKKK self-associate to form bilayer delimited...

Abstract: Peptide-based packaging systems show great potential as safer drug delivery systems. They overcome problems associated with lipid-based or viral delivery systems, vis-a-vis stability, specificity, inflammation, antigenicity, and tune-ability. Here, we describe a set of 15...

Abstract: Branched amphiphilic peptide capsules (BAPCs) are an efficient transport system that can deliver nucleic acids, small proteins, and solutes. The ability of BAPCs to break down is essential to their adoption as a delivery vehicle...

Abstract: Over the past decade, peptides have emerged as a new family of potential carriers in gene therapy. Peptides are easy to synthesize and quite stable. Additionally, sequences shared by the host proteome are not expected...

Abstract: Success for gene therapy clinical protocols depends on the design of safe and efficient gene carriers. Nature had already designed efficient DNA or RNA delivery devices, namely virus particles. However, they have a propensity to...

Abstract: Branched amphiphilic peptide capsules (BAPCs) are peptide nano-spheres comprised of equimolar proportions of two branched peptide sequences bis(FLIVI)-K-KKKK and bis(FLIVIGSII)-K-KKKK that self-assemble to form bilayer delimited capsules. In two recent publications we described the lipid...

Abstract: Understanding cellular uptake mechanisms of nanoparticles with therapeutic potential has become critical in the field of drug delivery. Elucidation of cellular entry routes can aid in the dissection of the complex intracellular trafficking and potentially...

Abstract: Objectives Primary: To evaluate knockdown of target protein following antisense siRNA delivery to GFP-CHO cells with BAPC versus current commercial transfection reagents. Secondary: To assess cellular viability through cryopreservation following BAPC treatment. Experimental design Transfection...

Abstract: Development of new and specific insect pest management methods is critical for overcoming pesticide resistance and collateral off-target killings. Gene silencing by feeding dsRNA to insects shows promise in this area. Here we described the...

Abstract: We recently reported on a new class of branched amphiphilic peptides that associate with double stranded DNA and promote in vitro transfection of eukaryotic cells. In the present study, we tested a different formulation in...

Abstract: Nanoparticles (NPs) have been shown to be a suitable mRNA delivery platform by conferring protection against ribonucleases and facilitating cellular uptake. Several NPs have succeeded in delivering mRNA intranasally, intratracheally, and intramuscularly in preclinical settings. 

Abstract: To eliminate the need for a cold chain, DNA vaccine approach was used, in which nanoparticles composed of branched amphiphilic peptide capsules (BAPC) were employed as the DNA delivery agent. The candidate vaccine and the...

Abstract: To test the transdermal delivery capabilities of BAPC-MB, the tails of live mice were dipped in BAPC-MB solution for 1, 5, 15 and 30 minutes. After a 24-hour incubation period, the tissues were harvested for...

Abstract: Introduction This report outlines two separate toxicity studies conducted on Apis melifera. The first study follows the parameters of the EPA Litmus test and the second study delves deeper into possible BAPC toxicity surpassing the...

Abstract: The ability of branched amphipathic peptide capsules (BAPC) to encapsulate and transport payloads into cells offers new approaches to deliver active ingredients (AIs). Until now, we have found that the BAPC were completely inert in...

Abstract: Branched Amphipathic Peptide Capsule Magnetic Beads (BAPC-MB) are a new nanoparticle developed for use as in diagnostic platforms. The bases are Maleimide Super Mag Magnetic Beads which are dispersed in 75% ethanol and combined with...

Abstract: To evaluate the effect of BAP encapsulation of magnetic nanobeads on both cellular uptake and cell viability, research was conducted with Branched Amphipathic Peptide Capsule Magnetic Beads (BAPC-MB) and rat intestinal epithelial cells (IEC-18). The...