BAPC-assisted-CRISPR-Cas9 Delivery into Adult Psyllid Ovaries and Pupae for Heritable Gene Editing (Hemiptera)

A new method BAPC-assisted-CRISPR-Delivery System, improved heritable gene editing when injecting adults or nymphs of insects [Hemiptera]. Addition of Branched Amphiphilic Peptide Capsules, BAPCtofect™ (Phoreus™ Biotechnology, Inc.,) improved delivery of CRISPR components for heritable gene editing, and also plasmids, or dsRNA for expression and gene targeting in insect nymphs and adults (Asian Citrus Psyllid- Diaphorina citri ). The method bypasses traditional microinjection of eggs.