Evaluation of transfection efficacy, biodistribution, and toxicity of branched amphiphilic peptide capsules (BAPCs) associated with mRNA
Abstract: Nanoparticles (NPs) have been shown to be a suitable mRNA delivery platform by conferring protection against ribonucleases and facilitating cellular uptake. Several NPs have succeeded in delivering mRNA intranasally, intratracheally, and intramuscularly in preclinical settings.Continue Reading
A Study of the Cellular Uptake of Magnetic Branched Amphiphilic Peptide Capsule
Abstract: Understanding cellular uptake mechanisms of nanoparticles with therapeutic potential has become critical in the field of drug delivery. Elucidation of cellular entry routes can aid in the dissection of the complex intracellular trafficking and potentially...Continue Reading
Peptiplexes – A Review of Therapeutic Applications. In “Nucleic Acid Nanotheranostics: Biomedical Applications
Abstract: This chapter will discuss cationic polymers containing ɛ-amine groups and immine or amide linkages that are being used as nanocarriers for nonviral gene delivery. We will focus particularly on cationic peptides. Cationic peptides, free or complexed...Continue Reading
BAPC-assisted-CRISPR-Cas9 Delivery into Adult Psyllid Ovaries and Pupae for Heritable Gene Editing (Hemiptera)
Abstract: A new method BAPC-assisted-CRISPR-Delivery System, improved heritable gene editing when injecting adults or nymphs of insects [Hemiptera]. Addition of Branched Amphiphilic Peptide Capsules, BAPCtofect™ (Phoreus™ Biotechnology, Inc.,) improved delivery of CRISPR components for heritable gene...Continue Reading
Germline Mutagenesis of Nasonia Vitripennis Through Ovarian Delivery of CRISPR-Cas9 Ribonucleoprotein
Abstract: CRISPR/Cas9 gene editing is a powerful technology to study the genetics of rising model organisms, such as the jewel wasp Nasonia vitripennis. However, current methods involving embryonic microinjection of CRISPR reagents are challenging. Delivery of Cas9...Continue Reading
Biodegradable Drug-Delivery Peptide Nanocapsules
Abstract: Branched amphiphilic peptide capsules (BAPCs) are an efficient transport system that can deliver nucleic acids, small proteins, and solutes. The ability of BAPCs to break down is essential to their adoption as a delivery vehicle...Continue Reading
Synthesis and Characterization of Multifunctional Branched Amphiphilic Peptide Bilayer Conjugated Gold Nanoparticles
Abstract: We provide strong chemical and biophysical evidence that documents that branched amphiphilic peptides, BAPs, known to assemble into spherical nanoassemblies in solution, do assemble as peptide-bilayer-delimited capsules. These nanoassemblies are termed branched amphiphilic peptide capsules...Continue Reading
Delivery of Lethal dsRNAs in Insect Diets by Branched Amphiphilic Peptide Capsules
Abstract: Development of new and specific insect pest management methods is critical for overcoming pesticide resistance and collateral off-target killings. Gene silencing by feeding dsRNA to insects shows promise in this area. Here we described the...Continue Reading
Branched Amphipathic Peptide Capsules: Different Ratios of the Two Constituent Peptides Direct Distinct Bilayer Structures, Sizes, and DNA Transfection Efficiency
Abstract: Branched amphipathic peptide capsules (BAPCs) are biologically derived, bilayer delimited, nanovesicles capable of being coated by or encapsulating a wide variety of solutes. The vesicles and their cargos are readily taken up by cells and...Continue Reading
Organization and Structure of Branched Amphipathic Oligopeptide Bilayers
Abstract: A class of self-assembling branched amphiphilic peptide capsules (BAPCs) was recently developed that could serve as a new drug delivery vehicle. BAPCs can encapsulate solutes up to ∼12 kDa during assembly, are unusually stable, and...Continue Reading
Rat Intestinal Epithelial Cells (IEC-18) Internalize Magnetic Nanobeads (MNBs) Encapsulated Within a Branched Amphipathic Peptide (BAP) BilayerView Data
Physical Characteristics of 50 and 200 nm Magnetic Beads when Encapsulated with Branched Amphiphilic Peptides (BAP)View Data
New Nanocarrier Technology Promises Breakthrough in Delivery of Therapeutic Drugs Through Mucosal MembranesRead the Article
Phoreus Biotech launches BAPtofect®-25 peptide transfection reagent as a superior alternative to industry-leading, lipid-based reagentsRead the Article
Phoreus Biotech Raises $2 Million in Seed Round for Development of New Genetic Vaccines and TherapeuticsRead the Article
Phoreus Biotech wins Animal Health Innovation USA Innovation Showcase for Production AnimalsRead the Article
What are Branched Amphipathic Peptide Capsules (BAPC®️)?
BAPC®️ are nanospheres made from two branched peptides that self-assemble to form solvent-filled, bilayer defined capsules. They serve as nano-delivery vehicles that overcome many of the poor absorption issues that plague certain classes of drugs: vaccines, therapeutics, diagnostics, pesticides, etc.
Due to their extreme temperature stability, BAPC have distinct advantages over other nano-carriers and can even be autoclaved. While liposomal-based carries break down too quickly in biological systems, polymer-based carriers don’t decompose at all and can build up in the environment. BAPC®️ offer just the right amount of stability while maintaining low cytotoxicity and immunogenicity.
How small are BAPC®️?
BAPC®️ are produced to specification in sizes ranging from six nanometers to two micrometers.
Why would I need different size BAPC®️?
The optimal size needed depends on the application. For diagnostic work, six nanometer BAPC®️ are ideal for gold or biomarker encapsulation. For cell transfection research, BAPC®️ are available in the following sizes: 6, 10, 25, and 50 nanometers. These sizes are ideal for achieving maximum transfection rates. Larger BAPC®️ can be prepared and can be used for encapsulating larger volumes for delivery of active ingredients such as fungicides.
How is the surface charge affected by addition of nucleic acid particles (i.e. iRNA and dsRNA)?
The positive surface charge is decreased due to electrostatic attractions but not to a point where their net charge becomes negative
Can BAPC®️ be customized for my specific application?
Yes. We realize that all research has unique considerations, so we can customize BAPC®️ to your exact specifications. If you’re not sure which size BAPC®️ are best suited for your research, we invite you to contact our team for a consultation.
What components can BAPC®️ be used with?
The composition of BAPC®️ allows hydrophilic small molecules, proteins and nucleic acids to be either surface bound or encapsulated within its compartment during the assembly process. This works both in vitro and in vivo.
What can BAPC®️ encapsulate?
- Fluorescent Dyes
- Therapeutic Drugs
- Colloidal Gold
- Magnetic Beads
What can be bound to the surface of BAPC®️?
- CRISPR-Cas9 components
Are BAPC®️ toxic?
When it comes to nanoparticles, there are a lot of unknowns and misconceptions in the industry. With much related research still in its infancy, the long-term effects of these new nanoparticles on human health and the environment are still being investigated.
While many nanoparticle carriers are toxic to target cells, BAPC®️ are non-toxic due to their natural peptide composition, stability and slightly positive surface charge. They also have the advantage of being non-immunogenic and completely biodegradable in the environment.
What commercial applications can BAPC®️ be used for?
BAPC®️ are an adaptable carrier platform with applications for multiple industries.
- Medical Vaccines
- Medical Therapeutics
- Diagnostic Imaging
- Veterinary Vaccines
- Veterinary Therapeutics
- Veterinary Pesticides
- Plant Nutrition
- Plant Insecticides
- Plant Fungicides
- Plant Herbicides
- Household and Commercial Biopesticides
- Premise Pest Control
- Cell Transfection
What is BAPtofect®️-25?
BAPtofect®️-25 kits are 25 nm conformationally locked BAPC in quantities of 0.5mg, 1.0 mg, 5 mg and 10 mg for use in cell transfection research. 25 nm BAPC®️ have been proven to be the ideal size to achieve maximum cell transfection rates.
How does BAPtofect®️-25 work for cell transfection?
Compared to the liposomal and polymer-based cell transfection materials available today, BAPC ®️offer an improved option for cell transfection research. In studies using HeLa and HEK-293 cells, BAPtofect®️-25 compared to an industry leading liposomal carrier yielded transfection rates that exceeded the cationic lipid with considerably less cytotoxicity.
BAPC®️ enter cells via the endosomal pathway providing targeted, protected delivery of their payloads. Both surface-bound proteins and nucleic acids are shielded from lytic degradation.
What is the surface charge of BAPtoFect®️-25?
+25 to 30 mV
Shipping and Storage
How is BAPC®️ shipped?
US shipments are sent via USPS Priority Mail for delivery within one to three days. International shipments are sent via Federal Express Economy unless expedited delivery is requested.
How is BAPC®️ stored?
BAPC®️ should be stored in a cool and dry location. BAPC®️ with added fluorescent dye should also be stored in a dark location.
What Our Users Say
“BAPtofect-25 systems have revolutionized our transfection process and allowed our undergraduate researchers to transfect dsRNA within the first week of being in our lab. Amazingly easy and accurate.”Dr. James Balthazor
Assistant Professor, Department of Chemistry
Fort Hays State University
“There was no difference in siRNA knockdown of a target protein in CHO cells following transfection with BAPC vs. two other transfection agents (TransIt-X2 and Lipofectamine 3000). In addition, we found that the presence of BAPC did not affect cell viability after one month of cryopreservation. We have plans to expand these comparisons to additional cell lines in the near future.”Dr. Barry Bradford
Professor, Animal Sciences and Industry
Kansas State University
“This technology will enable future medical and therapeutic tools. It carries infinite possibilities.”Dr. Yoonseong Park
Professor, Department of Entomology
Kansas State University
“The addition of BAPC improves delivery of CRISPR components, plasmids, and dsRNA for heritable gene editing and gene targeting in insect nymphs and adults. The first heritable gene Knockouts, using BAPC-assisted-CRISPR-Cas9, produced G2 mutants from injected adult females.”The FASEB Journal, Abstract Number: 626.2
BAPC-assisted-CRISPR-Cas9 Delivery into Nymphs and Adults for Heritable Gene Editing
Dr. Wayne Hunter