A Study of the Cellular Uptake of Magnetic Branched Amphiphilic Peptide Capsule
Understanding cellular uptake mechanisms of nanoparticles with therapeutic potential has become critical in the field of drug delivery. Elucidation of cellular entry routes can aid in the dissection of the complex intracellular trafficking and potentially…
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Branched Amphiphilic Peptide Capsule Uptake by Aspergillus Nidulans
The ability of branched amphipathic peptide capsules (BAPC) to encapsulate and transport payloads into cells offers new approaches to deliver active ingredients (AIs). Until now, we have found that the BAPC were completely inert in…
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Branched Amphipathic Peptide Capsules: Different Ratios of the Two Constituent Peptides Direct Distinct Bilayer Structures, Sizes, and DNA Transfection Efficiency
Branched amphipathic peptide capsules (BAPCs) are biologically derived, bilayer delimited, nanovesicles capable of being coated by or encapsulating a wide variety of solutes. The vesicles and their cargos are readily taken up by cells and…
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Gene Delivery and Immunomodulatory Effects of Plasmid DNA Associated with Branched Amphiphilic Peptide Capsules
We recently reported on a new class of branched amphiphilic peptides that associate with double stranded DNA and promote in vitro transfection of eukaryotic cells. In the present study, we tested a different formulation in…
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Branched Amphiphilic Cationic Oligopeptides Form Peptiplexes with DNA: A Study of Their Biophysical Properties and Transfection Efficiency
Over the past decade, peptides have emerged as a new family of potential carriers in gene therapy. Peptides are easy to synthesize and quite stable. Additionally, sequences shared by the host proteome are not expected…
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Synthetic In Vitro Delivery Systems for Plasmid DNA in Eukaryotes
Success for gene therapy clinical protocols depends on the design of safe and efficient gene carriers. Nature had already designed efficient DNA or RNA delivery devices, namely virus particles. However, they have a propensity to…
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Branched Amphiphilic Peptide Capsules: Cellular Uptake and Retention of Encapsulated Solutes
Branched amphiphilic peptide capsules (BAPCs) are peptide nano-spheres comprised of equimolar proportions of two branched peptide sequences bis(FLIVI)-K-KKKK and bis(FLIVIGSII)-K-KKKK that self-assemble to form bilayer delimited capsules. In two recent publications we described the lipid…
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